The occurrence, abundance and distribution of microplastics (MPs) were studied in sediments and surface waters of the Anzali coastal wetland, southwest of the Caspian Sea. Sediment and surface water samples were taken at 11 and 6 stations in June 2018 and January 2019 respectively. The abundances of MPs in sediment samples were 140–2820 and 113–3690 items/kg dry weight while in surface waters were 0.40–4.41 and 0.19–2.85 items/m3 in June and January respectively. Fiber was the most common shape, followed by fragment and film. Red, black and blue were the most frequent colors. Particles 1–2 mm were the dominant size. SEM/EDS and ATR-FTIR analyses were used to identify MPs. Polypropylene, polyethylene and polyester were the most frequently found polymers. Our results highlighted the widespread dispersion of MPs in Anzali Wetland which in turn might be a pathway of MPs pollution transporter to the adjacent and connected Caspian Sea.