L-asparaginase enzyme, which is widely available in nature, is used in combination with other drugs in the treatment of acute lymphocytic leukemia, lymphosarcoma, melanosarcoma and various types of cancer [1]. This enzyme also plays an important role in the food industry and as a food processing agent in reducing the level of acrylamide formation (a carcinogenic compound) during the production of starchy food products[2]. Medicinal plants are among the natural resources that are used in traditional and modern medicine to treat human diseases [3]. In this study, the kinetic parameters of L-asparaginase enzyme extracted from cow liver in the presence of oregano plant alkaloid extract were investigated.