محمد حسین فاطمی

Static headspace coupled to gas chromatography-mass spectrometry (SHS-GC-MS) was applied to the extraction and analysis of volatiles in some Iranian rice varieties. Optimization of static headspace sampling was investigated by full factorial design and a face-centered central composite design procedure. According to the results of full factorial design, the effective parameters of static headspace sampling were extraction temperature (T) and extraction time (t). Then a central composite design was applied to find the optimum level of these factors. The results of three replications of experiments under optimal conditions (T=100oC, t=900s) indicated that, there is a good agreement between the predicted (5.28) and experimental (5.20) responses (98.48%). Then developed method was used for analysis of volatile chemicals in three Iranian rice cultivars. According to the results of GC-MS analysis 29, 24 and 22 chemicals were assigned to Domsiah, Deylamani and Tarom rice samples, respectively. The major compounds identified in these samples were the alkyl aldehydes. The results obtained are promising for future isolation and identification of the flavor volatiles of other rice cultivars by SHS-GC-MS analysis.