ABSTRACT Herein, Polymethyldopamin@Fe3O4 (PMDP@Fe3O4) has been synthesized using a very simple, easy, cost-effective, efficient, and fast method. First, magnetic nanoparticles (Fe3O4) were synthesized. It was followed by accommodating polymethyldopamin (PMDP) on the prepared Fe3O4. The prepared PMDP@Fe3O4 was utilized as a sorbent in the Magnetic Solid Phase Extraction (MSPE) of six selected Polycyclic Aromatic Hydrocarbons (PAHs) including anthracene, fluoranthene, fluorene, naphthalene, phenanthrene, and pyrene. The extracted model analytes were desorbed by a Deep Eutectic Solvent (DES) and were analyzed by High-Performance Liquid Chromatography-Ultraviolet detection (HPLC-UV). The method is validated according to ICH guidelines with respect to precision, accuracy, linearity, specificity, robustness, and limits of detection and quantification. Under the optimized condition, the linearity of the method was in the range of 0.1–500 mg L1 for the selected analytes. The calibration curves showed a determination of coefficient (r2) higher than 0.9986. The limits of detection (LODs) and limits of quantification (LOQs) were calculated to be between 0.03–0.14 and 0.09–0.46 mg L1, respectively. The inter-day and intra-day relative standard deviations (RSDs%) were determined to be in the range of 1.7–4.2% in three concentrations. Furthermore, the enrichment factors were found to be 76, 61, 65, 78, 69, and 67 for anthracene, fluoranthene, fluorene, naphthalene, phenanthrene, and pyrene, respectively. Moreover, the calculated absolute recoveries were between 61 and 78%. The results of the real sample analysis and the obtained recoveries indicated that the method was useful and applicable in complicated real samples.
