A novel biomaterial based on hydrolyzed pectin(HP)/polyaniline(PA)/graphene oxide(GO) has been prepared via chemical polymerization method. The SEM analyses revealed a rod-like and laminated morphology for NH-mGO@HP and NH-mGO@HPPA nanocomposites. Higher thermal stability for NH-mGO@HP-PA was obtained than other samples. Biosensor was designed via electrodeposition of NH-mGO@HP-PA nanocomposite on carbon graphite electrode. Biosensor activity showed a considerable increase in oxidation current by the nanocomposite. The NH-mGO@HP-PA indicated higher electrical conductivity compared to pure PA.