Herein, the authors report the synthesis of electro-magnetic polyfuran/Fe3O4 nanocomposites using Fe3O4 magnetic nanoparticles of different content as nucleation sites via in situ chemical oxidation polymerization method. Surface, structural, morphological, thermal, electrical and magnetic properties of the nanocomposites were studied by FT-IR, UV-visible spectroscopies, XRD, FESEM, TGA, four probe, and VSM, respectively. The effect of Fe3O4 nanoparticles content on the electrical conductivity and magnetization of nanocomposites was studied. The obtained polyfuran and polyfuran/Fe3O4 nanocomposites were analyzed for their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. In addition, polyfuran/Fe3O4 nanocomposites have been investigated for application as electrochemical biosensor.