The present work describes a label free electrochemical aptasensor for selective detection of epirubicin. In this project, 5′-thiole terminated aptamer was self-assembled on carbon screen printed electrode, which modified with electrodeposited gold nanoparticles on magnetic double-charged diazoniabicyclo [2.2.2] octane dichloride silica hybrid (Fe3O4@SiO2/DABCO) by Au-S bond. The interactions of epirubicin with aptamer on the AuNPs/Fe3O4@SiO2/DABCO/SPE have been studied by cyclic voltammetry, linear sweep voltammetry and electrochemical impedance spectroscopy. Under optimized conditions, the peak current of epirubicin increased linearly with increasing epirubicin concentration, due to the switching in the aptamer conformation and formation of aptamer- epirubicin complex instead of aptamer on the modified electrode surface. The Apt/AuNPs/Fe3O4@SiO2/DABCO/SPE is sensitive, selective and has two linear ranges from 0.07µM to 1.0 µM and 1.0 µM to 21.0µM with a detection limit of 0.04 µM. The applicability of the aptasensor was successfully assessed by determination of epirubicin in a human blood serum sample.
