Electrochemical DNA biosensors are particularly attractive because of high sensitivity, suitability and compatibility in miniaturization in the nucleic acid technology. In this course, different kind of biosensors could be developed to study different aspects of life science as diseases, mutagenious, and the way of interaction between ligands, drug and molecules to DNA. In this study, the electrochemical biosensor was prepared by modifying screen-printed graphite electrode with mesoporous structure of MCM41 to immobilize short sequence of p53 tumor suppressor gene. The mesoporous structure introduces a significant surface area and can increase the amount of adsorbed DNA on the surface. The interaction of flutamide as the anticancer drug with ssDNA and dsDNA was studied in Tris-HCl buffer solution and also in the presence of Hemin as a suitable electroactive redox label. The differential pulse voltammetry current of HEM reduction peak decreases with the increasing concentrations of Flu due to the interaction of DNA/Flu. The peak current of HEM was linearly decreased with the concentration of Flu in the range of 0.7 to 10 μM (R2 = 0.99), with a detection limit of 0.1 μM.
