A carbon-paste electrode modified with 2,7-bis(ferrocenyl ethyl)fluoren-9-one and carbon nanotubes was used for the sensitive voltammetric determination of levodopa (LD). The electrochemical response characteristics of the modified electrode toward LD, uric acid (UA) and folic acid (FA) were investigated. The results showed an efficient catalytic activity of the electrode for the electrooxidation of LD, which leads to lowering its overpotential by more than 320 mV. The modified electrode exhibits an efficient electron mediating behavior together with well-separated oxidation peaks for LD, UA and FA. Also, the modified electrode was used for determination of LD in some real samples.