In this project, a three-dimensional graphene oxide coated agarose/chitosan (ACGO) porous film was syn- thesized and utilized as sorbent in thin film microextraction (TFME) technique to extract 4-chlorophenol, 2,4-dichlorophenol, 3,5-dichlorophenol and 2,4,6-trichlorophenol as the model analytes in various real samples such as agricultural waste water, honey and tea samples. In addition, deep eutectic solvent made of tetra ethyl ammonium chloride/chlorine chloride was used as a desorption solvent. The ef- fect of various variables, such as: extraction time, stirring rate, solvent desorption volume, desorption time, ionic strength and solution pH on the extraction efficiency of the method was studied and op- timized. Under the optimized condition, the linear range of the method was obtained in the range of 0.1–500 μg L −1 for testing analytes (4-chloropheol = 0.1-500 μg L −1 , 2,4-dichlorophenol = 0.2-500 μg L −1 , 3,5-dichlorophenol = 0.5-500 μg L −1 and2,4,6-trichlorophenol = 0.2-500 μg L −1 ). Theobtainedcorrelation coefficients ( r 2 ) were between 0.9984 and 0.9994. The limits of detection (LODs) were also calculated between 0.03 - 0.13 μg L −1 . The relative standard deviations (RSDs%) were obtained in the range of 2.8 to 5.9%. The enrichment factor (EFs) values for the studied analytes were also obtained in the range of 33.4-35.8. In addition, the obtained results indicated that the prepared film can potentially be used for more applications in the field of environment, food safety, and drug analysis.
