1403/01/10
جهانبخش رئوف

جهانبخش رئوف

مرتبه علمی: استاد
ارکید:
تحصیلات: دکترای تخصصی
اسکاپوس:
دانشکده: دانشکده شیمی
نشانی:
تلفن: 01135302392

مشخصات پژوهش

عنوان
Label-free and sensitive aptasensor based on dendritic gold nanostructures on the functionalized SBA-15 for determination of chloramphenicol
نوع پژوهش
JournalPaper
کلیدواژه‌ها
Aptamer; Chloramphenicol; SBA-15@DABCO.Hemin; Dendritic gold nanostructures; Screen-printed graphite electrode
سال
2016
مجله ANALYTICAL AND BIOANALYTICAL CHEMISTRY
شناسه DOI
پژوهشگران Ayemeh Baghery Hashkavayi ، Jahan Bakhsh Raoof ، Razieh Azimi ، Reza Ojani

چکیده

A highly sensitive and low-cost electrochemical aptasensor was developed for the determination of chloramphenicol (CAP). The system was based on a CAP-binding aptamer, a molecular recognition element, and 1,4-diazabicyclo[2.2.2]octane (DABCO)-supported mesoporous silica SBA-15 on the surface of a screen-printed graphite electrode for formation of dendritic gold nanostructures and improving the performance and conductivity of the biosensor. Hemin has been applied as an electrochemical indicator which interacted with the guanine bases of the aptamer. In the absence of CAP, hemin binds to the aptamer and produces a weak differential pulse voltammetric (DPV) signal. The presence of CAP led to stabilization of the folded aptamer, which generated an amplified DPV signal. The peak current of hemin increased linearly with the concentration of CAP. Under optimal conditions, two linear ranges were obtained from 0.03 to 0.15μM and 0.15 to 7.0μM, respectively, and the detection limit was 4.0 nM. The prepared biosensor has good selectivity against other non-target drugs. Thus, the sensor could provide a promising platform for the fabrication of aptasensors. The feasibility of using this aptasensor was demonstrated by determination of CAP in a human blood serum sample.