In this paper, we consider an inverse reaction–diffusion–convection problem in which one of the boundary conditions is unknown. A sixth-kind Chebyshev collocation method will be proposed to solve numerically this problem and to obtain the unknown boundary function. Since this inverse problem is generally ill-posed, to find an optimal stable solution, we will utilize a regularization method based on the mollification technique with the generalized cross-validation criterion. The error estimate of the numerical solution is investigated. Finally, to authenticate the validity and effectiveness of the proposed algorithm, some numerical test problems are presented.