Dextran ultra-fne nanofber mats with uniform morphological appearance were fabricated using the electrospinning process. Increasing the solution concentration from 0.5 g.mL−1 to 0.9 g.mL−1 changed the fber structure from coarse-beaded to a uniform beadless nanofber mats. Additionally, reducing the feed rate from 1 mL.h−1 to 0.25 mL.h−1 with increasing the spinning distance from 150 to 170 mm generally led to a decrease in the diameters of the fbers. At the end; uniform dextran nanofbers with diameters ≈227 nm fabricated by the systematic modifcation of the electrospinning process. Crosslinked dextran nanofber mats represent promising candidates for biomedical and tissue engineering.