Surfactant-assisted dispersive liquid-liquid microextraction followed by high-performance liquid chromatography with uv detection is a green microextraction that has been developed for the preconcentration and trace detection of celecoxib in human plasma. in this method, the extraction is carried out by forming the ion pair between analytes and surfactant in the sample solution and transferring it to a low density solvent. A conventional cationic surfactant called cetyltrimethyl ammonium bromide (CTAB) was use as a disperser agent in the proposed approach. 0.5mL of CTAB (0.09 mmolL -1) (disperser solvent) containing 40.0μL of 1-undecanol (extraction solvent) was injected rapidly into the 9.0mL of diluted plasma. A cloudy solution was formed in the test tube. After formation of cloudy solution, the mixture was centrifuged and 20μL of collected phase was injected into HPLC for subsequent analysis [1]. The use of surfactant (Emulsifier) and low density solvent (extraction solvent), which have a low toxicity, is one of the benefits of this method, which will provide the best recovery in the shortest possible time and making it an environmentally friendly method. The predominant parameters affecting extraction efficiency such as the type and volume of extraction solvent, the type and concentration of surfactant, sample pH and the concentration of salt added to the sample were investigated and opted [2]. Under the optimum conditions (extraction solvent and its volume, 1-undecanol, 40μL; surfactant and its concentration, 0.5 mL of ultra-pure water containing 0.09 mmolL-1 cetyltrimethyl ammonium bromide; sample pH = 10 and salt content of 4% NaCl w/v), the extraction recovery was 96%. The proposed method was successfully applied for preconcentration of the drug in plasma sample with the enrichment factor of 340 and the method showed good linearity range of 1-500μgL−1 (R 2> 0.9981). The validated method showed a satisfactory performance and can be served as a simple and sens
