Abstract Naringenin is one of the most important flavonoids with antioxidant property which exists in abundance in grapefruit. Regarding to the effective role of naringenin in prevention of diseases such as cancer and hematite C, extraction and determination of this compound is very important. In this work, a sensitive, rapid and efficient method for the extraction of naringenin as well as its determination in grapefruit sample was developed using magnetic stirring assisted dispersive liquid–liquid microextraction (MSA-DLLME) followed by HPLC-UV. The extraction method is based on the application of an extraction solvent lighter than water in the ternary component solvent (aqueous solution: extraction solvent: disperser solvent) system. The experimental factors affecting the extraction recovery including type and volume of extraction and disperser solvents, pH of sample solution, salt addition and extraction time on extraction recovery were optimized. Under the optimum extraction condition (100 μL 1-octanol as the extraction solvent, 300 μL methanol as the disperser solvent, pH of the sample solution = 4.5 and no addition of NaCl to the sample solution), limit of detection, the extraction recovery and enrichment factor were 0.3 ng mL-1, 86% and 62, respectively; and the obtained linear range was between 1 ng mL-1 and 700 ng mL-1 (R2 = 0.9993). The method was successfully applied for the preconcentration and determination of naringenin in grapefruit sample.
