In this work modified Cellulose nanofibers was applied for extraction of Secondary Metabolites from Portulaca Olercea). Herbs of Portulaca Olercea were harvested in the growing culture of Babolsar, Mazandaran, IRAN. After the powdering 0.1 g of the leaves, vortex assisted solvent extraction was used to prepare the extracts. Then a chemometrics approach was applied for the optimization of the extraction parameters such as Sorbent mass, volume and concentration of aqueous solution of CTAB, percentage and type of acid and vortex time. The extraction yield acted as the comprehensive evaluation index. Total phenolic compounds, total flavonoid compounds, and antioxidant activity were used to evaluate these extracts. The individual phenolic compounds found in the extracts were characterized and quantified by HPLC. The Portulaca Olercea plant extract showed the highest total phenolic and flavonoid values, at 87.423 mg GAE/g and 94.461 mg RE/g, respectively. The HPLC results have shown that the levels of Gallic acid, Proto-catechuic acid, Chlorogenic acid, Rosmarinic acid, Caffeic acid, Syringic acid, Luteolin, Ferulic acid and Apigenin were as follows: 4.3, 5.9, 8.5, 14.1, 16.7, 17.8, 18.5, 19.6, 19.8 and 21.4 mg/g, respectively. Under the optimum conditions, the values of intra and inter day RSD were in the range of 3.91–4.96% and 5.91–8.98%, respectively. The limits of detection (LODs) ranged between 4.02 - 8.64 ngmL-1 respectively. The method was successfully applied for the preconcentration of phenolic compounds in 91.006 mg GAE/g leaves. Moreover, these experimental values were higher than those obtained from previously reported. This study suggests that modified Cellulose nanofibers can be utilized as sustainable and safe extraction method for natural product extractions.
