Simulation, design and fabrication of a sealing enclosure is carried out for a NaI(Tl) 2″×2″ detector, to be used as in situ gamma radioactivity measurement system in marine environment. Effect of sealing enclosure on performance of the system in laboratory and marine environment (distinct tank with 10 m3 volume) were studied using point sources. The marine volumetric efficiency for radiation with 1461 keV energy (from 40K) is measured with KCl volumetric liquid source diluted in distinct tank. The experimental and simulated efficiency values agreed well. Marine volumetric efficiency calibration curve is calculated for 60 keV to 1461 keV energy with Monte Carlo method. This curve indicates that efficiency increasing rapidly up to 140.5 keV but then drops exponentially.