In this research, the effects of cold plasma treatment on the properties of gelatinbased emulsion films (GEFs) using different gases were investigated. The gases used include O2, N2, air, Ar, and ethanol-argon (EtOH-Ar). Surface hydrophobicity, morphology, water vapor permeability (WVP), and mechanical, thermal, and antifungal properties after plasma application on the film were analyzed. The results revealed that surface hydrophilicity significantly increased after cold plasma, while the contact angle significantly decreased (p < .05). Furthermore, atomic force microscopy results showed that the argon gas plasma significantly increased roughness of the GEFs surface. Besides, plasma did not decrease WVP. Different gases had no significant effect on the mechanical properties of the GEFs (p > .05). Oxygen permeability after plasma application was significantly different from the control sample; consequently, the permeability after plasma application decreased and the lowest level 55.7 (cm3 μm m−2 day−1 Pa−1) was seen for oxygen gas. Plasma treatment caused etching effects and lessened the surface moisture of the polymer film. Antimicrobial activity was observed in the cold plasma-treated samples, especially under air and nitrogen atmosphere. Cold plasma treatment is an effective method for surface modification, expanding the application of emulsion films in the packing industry with improved performance properties