1403/01/09

فرشته میر محمدرضایی

مرتبه علمی: استادیار
ارکید:
تحصیلات: دکترای تخصصی
اسکاپوس:
دانشکده: دانشکده علوم پایه
نشانی: گروه زیست شناسی ،دانشکده علوم پایه، دانشگاه مازندران
تلفن: 35302450

مشخصات پژوهش

عنوان
Effect of astaxanthin and melatonin on cell viability and DNA damage in human breast cancer cell lines
نوع پژوهش
JournalPaper
کلیدواژه‌ها
Astaxanthin,Melatonin,Bcl2,DNA damage,Breast cancer
سال
2022
مجله ACTA HISTOCHEMICA
شناسه DOI
پژوهشگران Fereshteh Mir Mohammadrezaei ، Aida karimian ، Mohammad hadi bahadori ، Akbar Hajizadeh Moghaddam ، Marjan Ghorbani-Anarkooli ، Asadollah Asadi ، Arash Abdolmaleki

چکیده

Background Astaxanthin is a xanthophyll pigment found in algae and marine animals, having strong anti-oxidative, anti-tumoral, and anti-inflammatory effects. Additionally, melatonin has shown inhibitory effects on the growth of human breast cancer cells. The aim of the present study was to evaluate the effect of astaxanthin and the combined effects of astaxanthin and melatonin on breast cancer cells and the non-tumoral breast cell line. Materials and methods The human breast cancer cell lines, T47D and MDA-MB-231, and non-tumorigenic cell line MCF 10A were treated and compared to astaxanthin, melatonin, and co-administration of these two compounds. Cell viability, apoptosis induction, Bcl-2 protein expression, and DNA damage were measured by MTT assay, acridine orange/ethidium bromide (AO/EB) staining, immunocytochemistry, and comet assay. Results Astaxanthin at lower doses than melatonin reduced cell viability and Bcl2 expression, induced apoptosis and DNA damage in MDA-MB-231 and T47D. Meanwhile, the effects of astaxanthin on cell cytotoxicity, apoptosis, and DNA damage in MCF10A cells are insignificant compared to MDA-MB-231 and T47D. Moreover, the results indicated that astaxanthin in T47D cells caused more cell death compared to MDA-MB-231 cells. Astaxanthin induced cell death on breast cancer cells and without cell cytotoxicity for non-cancerous cells. Conclusion Furthermore, the presence of astaxanthin increased the function of melatonin-induced cell death in breast cancer cells.