The present work explains the fabrication of a novel electrochemical aptasensor for identifying and measuring the epirubicin (Epi) by using curcumin (Cur) as an anticancer electrochemical indicator. The aptasensor prepared by immobilizing the thiolated aptamer on the surface of graphite screen-printed electrode modified with gold nanoparticles/functionalized multiwall carbon nanotubes, ionic liquid and chitosan nanocomposite (AuNPs/FMWCNTs-IL-Chit/SPE). To evaluate the willingness of aptamer to interaction with Epi in the presence of complementary strand DNA, competitive binding assay between the complementary strand of aptamer and Epi were used. Cur tends to bound to the grooves of two strands DNA. With increasing the concentration of Epi in the range of 0.007–7.0mM, the peak current of Cur decreased, due to the formation of aptamer-Epi complex and decreasing the amount of complementary strand DNA. Through the control experiments, we examined the response of fabricated aptasensor for some anticancer drugs, which have a structure similar to the Epi. The results showed that using the thiol-terminated aptamer as a recognition layer led to a sensor with a high tendency for Epi compared to other anticancer drugs.
