Prussian blue nanoparticles (PBNPs) were prepared by a self-assembly process on a glassy carbon electrode (GCE) modified with poly(o-phenylenediamine) (PoPD) film. The stepwise fabrication process of PBNP-modified PoPD/GCE was characterized using scanning electron microscopy and electrochemical impedance spectroscopy. The prepared PBNPs showed an average size of 70 nm and a homogeneous distribution on the surface of the modified electrode. The PBNPs/PoPD/GCE showed electrocatalytic activity towards the oxidation of pyridoxine (PN) and was used as an amperometric sensor. The modified electrode exhibited a linear response for PN oxidation over the concentration range 3–38.5 μM with a detection limit of ca 6.10× 107 M (S/N=3) and sensitivity of 2.79936 × 103 mAM1cm2 using an amperometric method. The mechanism and kinetics of the catalytic oxidation reaction of PN were investigated using cyclic voltammetry and chronoamperometry. The values of α, kcat and D were estimated as 0.36, 1.089 × 102 M1 s1 and 8.9 × 105 cm2 s1, respectively. This sensor also exhibited good anti-interference and selectivity. Copyright © 2016 John Wiley & Sons, Ltd.
