One of the major health clinical problems in patients with diabetes patients is impaired wound healing. Diabetic foot ulcer is a major complication of diabetes mellitus in 12 to 25% of patients, which increases the risk of damage in the limbs or amputation. The earthworm Eisenia foetida glycolipoprotein (as known G-90) is a blend of macromolecules with some biological properties including mitogenicity, anticoagulation, fibrinolysis, bacteriostatic and antioxidatiaon. Collagen components, such as fibroblast and keratinocytes, are a major part of skin development. Collagen type I is the main protein of the extracellular matrix (ECM) and critical for mechanical protection of tissues and organs. Due to health problems caused by mammalian collagen and religious barriers, use of collagen and collagen products is limited. Therefore, fish wastes such as skin, bone, scales, and fins are considered as alternatives to collagen sources. Given the biological properties of G-90 and acid soluble collagen (ASC) extracted from fish scale, this study is conducted to investigate the skin tissues fine structures of alloxan induced diabetic rats affected by extract obtained from the homogenate of Eisenia foetida (G-90) combined with ACS on the wound healing process. In this study, high photon flux and coherent beam properties can detect alteration in alloxan-induced diabetic rats structure and biochemical function in diabetic disease. We will use a fast imaging detector FPA coupled to the infrared microscope to map out large region of the diabetic rats skin sections, and help identify the relevant regions of interest before analyzing them with the synchrotron source.
