Impaired wound healing in diabetic foot ulceration is one of the severe challenges in diabetic patients, which profoundly affects their quality of life. Today, applying a novel and non-invasive method to promote wound healing in diabetic patients is of considerable interest. In this study, we aimed to load acid-soluble collagen (ASC) extracted from fish scale and the earthworm glycolipoprotein (G-90), ASC + G-90, and test it on injured diabetic rats. The purpose of this research was to investigate the novel effects of ASC + G-90 on wound healing by using the synchrotron radiation Fourier transform infrared (SR-FTIR) microspectroscopy. The results showed that oxidative stress and lipids peroxidation were least pronounced in treated skin with ASC + G-90 (T) compared with treated skin with panthenol-D (PC) and the skin without any treatment (NC). The collagen formation was notably observed in the dermis of treated group with ASC + G-90. These findings clearly confirmed the effectiveness of the treatment in skin repair.
