Multiple sclerosis (MS) is a chronic inflammatory demyelinating and neurodegenerative disease of the central nervous system characterized by unpredictable and variable clinical course. Etiology of MS involves both genetic and environmental factors. The environmental factors concerned are likely to be infectious agents, and the involvement of numerous viral agents in the etiology of MS has been postulated. The purpose of this study was to investigate the relationship between Torque Teno virus (TTV) and etiopathogenesis of MS, and its prevalence in this group of patients. Materials and Methods: Blood samples from 60 patients with MS and 30 healthy controls were collected. DNA extraction and real-time PCR based on (Syber Green) were done in the serum samples. Clinical data were collected and analyzed by SPSS version 21. Results: Despite the fact that all cases were positive in both the control group and the patient group, the mean Ct (cycle of threshold) of the two groups was different. The mean standard deviation of the patient group and the control group was 21.6 ± 9.73 and 30.3 ± 1.64, respectively. Comparison of the means using the independent samples t-test method in SPSS software showed that the difference in means between the patient and healthy groups is statistically significant. Conclusion: Based on the conducted research, it appears that factors involved in the development of MS may lead to increased replication of thetorque teno virus in these patients. However, further investigations are needed.
