Widespread use of Wi-Fi equipment, have persuaded scientists to design different experiments to investigate the hazardous consequences of long term exposure to Wi-Fi signals. Electromagnetic radiation emitted by such devices may have adverse impacts on various organs specially brain. Our aim is to investigate the consequence of exposure to electromagnetic waves and change in glutathione S-transferase (GST) activity of rat brain. For this purpose we prepared two groups of control and Wi-Fi exposed rats. Each group consisted of six rats. Exposure group were treated by radiation emitted from Wi-Fi equipment for 10 weeks. At the end of the experimental period, animals were anaesthetized; their brain tissues were extracted and homogenized with proper buffer. After wards GST activity was evaluated by Habig method using GSH and 1-chloro-2, 4-dinitrobenzene as substrates. There were significant increase in the mean GST activity in brain of Wi-Fi exposed rats compared with control group (3.54±0.34 Vs 4.37±0.76 U/g, P=0.038). GST play a pivotal role in the detoxication of the products of oxidative stress. In conclusion, the present findings suggest that exposure to electromagnetic fields may cause an increase in GST activity in brain in response to oxidative stress.