There is growing concern regarding the potential human health hazard due to exposure to radiofrequency electromagnetic radiation emitted from Wi-Fi equipment. Our study has been devoted to investigate the possible effects of electromagnetic radiation from Wi-Fi equipment on rat brain. For this purpose, the level of lipid peroxidation was determined by measuring of the content of malondialdehyde (MDA) in whole brain of rat exposed to Wi-Fi equipment. Twelve male rats were selected and divided into two groups for this study. One control group and other group exposed to radiation emitted from Wi-Fi equipment continuously for ten weeks. After passing exposure time whole brain tissue were homogenized with PBS 100mM, pH 7.4. MDA was determined using the thiobarbituric acid reactive substance assay, as described by Buege & Aust. A significant increase was observed in MDA content in brain of rats exposed to Wi-Fi equipment compared with control group (106.37±23.05 vs 137.18±21.13 ,P=0.036). It is obvious that MDA, a marker of oxidative injury of cellular membranes, significantly increased after 10 weeks exposure to Wi-Fi signals. The high concentration of MDA is a direct evidence of toxic processes caused by free radicals. Therefore, it can be concluded that wi-fi equipment caused oxidative stress and lipid peroxidation in brain tissue.