Introduction & Objectives: Wi-Fi equipment is being installed in household or many public places including schools, offices, hospital and libraries. There are concerned that electromagnetic radiation emitted from Wi-Fi equipment could cause health problems and testis tissue are more susceptible to such radiation. To our knowledge, very few studies, if any, have investigated the sensitivity of the reproductive system in users continuously exposed to radiation from Wi-Fi equipment. Materials & Methods: In our study 12 male rat, divided in two groups of control, were included in the current experiment. The experimental group were exposed to radiation from Wi-Fi equipment for 10 weak. After completing the exposure period, the rats were anaesthetized and its testis was dissected. Catalase activity was assayed according to method of Aebi using H2O2 as the substrate. Results: Analysis of testis indicated that catalase activity in Wi-Fi exposed group was significantly decreased compared with control group (310.27±92.79 Vs 571.07±53.84 U, P=0.04). Conclusion: Consistent with the effect of electromagnetic radiation on various organs, an increase in the damage to reproductive system were observed 20 weak following exposure to Wi-Fi radiation. The decreased activity of catalase, an important cellular antioxidant defense component, may lead to increased levels of prooxidants such as superoxide radicals and hydrogen peroxide. In conclusion, decreased activities of antioxidant enzymes in the individual exposed to radiation from Wi-Fi equipment can lead to oxidative stress in testis tissues, which might be responsible for the initiation or progression of various disorder.