In today's world, electromagnetic radiation-emitting devices particularly wireless access points are widely used in household, industrial, medical, military, and scientific applications. Electromagnetic radiation from Wi-Fi equipment appears to induce neurodegenerative processes through increase of oxidative stress and decrease of antioxidants in the brain. Our study has been devoted to the investigation of the possible effects of electromagnetic radiation from Wi-Fi equipment on rat brain. For this purpose, two group of rat involved in this study, one control group and other group exposed to radiation emitted from Wi-Fi equipment. Brain tissue was homogenized were homogenized with PBS 100mM, pH 7.4 in a tissue homogenizer (Ika-Ultra-Turrax T25, Germany). Total antioxidant capacity of rat brain were measured with the FRAP assay. Total antioxidant capacity level of rat brain was significantly increased in Wi-Fi exposed rats (560.50±93.25 Vs 409.50±67.85 nmol/g tissue, P<0.05). According to our results, the antioxidative defense system were attenuated in the brain of rat exposed to Wi-Fi radiation. In conclusion, exposure to Wi-Fi emitted radiofrequency radiation may cause brain oxidative damages. Future studies are warranted to identify the specific intracellular signaling pathways that transduce Wi-Fi-induced changes in the brain of exposed cases.