1403/01/10
علی طراوتی

علی طراوتی

مرتبه علمی: استادیار
ارکید:
تحصیلات: دکترای تخصصی
اسکاپوس:
دانشکده: دانشکده علوم پایه
نشانی:
تلفن: 35305250

مشخصات پژوهش

عنوان
Thiolation of Chitosan nanoparticle and immobilization of Laccase enzyme
نوع پژوهش
Presentation
کلیدواژه‌ها
Chitosan, Thiolated nanoparticle, Laccase, Enzyme immobilization
سال
2018
پژوهشگران Ali Taravati ، Fatemeh Tohidi ، Seyyedeh Tahereh Mirsalehi

چکیده

Laccase has a lot of importance in biotechnology and many industrial applications.Immobilized enzyme present enhanced activity and storage time, pH and thermal stability compared with free enzyme. In this study, Laccase enzyme immobilized onto the thiolated chitosan nanoparticle. The thiolated chitosan nanoparticle characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Chitosan nanoparticle was synthesized using sodium tripolyphosphate (TPP) as a polymerizing agent. Thiolated chitosan nanoparticle was prepared by adding thioglycolic acid (TGA). Finally, to performing immobilization, laccase enzyme (0.025 g/ml) loaded onto thiolated chitosan nanoparticle in the solution with pH of 5.5 by 24 hours incubation time at room temperature. Under these optimum circumstances, the efficiency of laccase immobilization was on the range of 42-48%. Based on our results, thiolated chitosan nanoparticle can be used as an ideal support for laccase immobilization in the biotechnological application.