Inulin a polysaccharide that is a mixture of fructose oligomers and polymers. This is a prebiotic sugar, which is found in More than 36000 types of plants In this research, Helianthus tuberosus L. is wild and tuberous, from the sunflower family, which is a source of Inulin sugar. In the present study, inulin is extracted from H. tuberosus populations of Guilan, Golestan and Mazandaran provinces and then the best population in terms of Inulin amount is introduced. After collection of tubers, 0.05 gr of each tuber was measured after that methanol extract was prepared by using 1.5 ml of potassium phosphate buffer. The samples were moved to ultrasonic waves for 10 minutes. In addition, for checking the sugar amount, Anthron method was used. Then the samples for 8 minutes in a water bath of 1 of total inulin and fructose were measured by spectrophotometry at wavelengths of 520 and 625 nm. The results showed that the levels of the sugar in studied populations are not similar and same. The highest amount of inulin absorbed in Ramsar (361.87 DW), Gorgan (336.37 DW) and Astaneh Ashrafieh (319.12 mg/g dry weight) populations. Several ecological factors including light intensity, number of sunny days per year, moisture content, and altitude and soil acidity have significant effects on creation of different amounts of inulin in all studied populations.
