Background: Recent studies have shown that reactive oxygen species (ROS) play a role in various types of male infertility and it is due to the over production of ROS or decrease in the antioxidant defense system in the reproductive system and sperm. In pathological conditions, ROS via interferences in the spermatogenesis process. These ROSs led to lipid peroxidation (LPO) and the establishment of firm peroxidation products a like malondialdehyde (MDA) in seminal and blood plasma. The purpose of this study was to investigate the association of two biomarkers of oxidative stress; total antioxidant capacity (TAC) and MDA with quality-quantity factors in alcoholic and smokers men Methods: Forty seminal samples including: 22 normal samples, as a control groups (non alcoholic and smokers men) and 18 alcoholic and smokers male infertility samples, as a case groups, were collected from the Fatemezahra IVF Centre (Babol, Iran). Seminal and blood plasma TAC and MDA levels in all samples were measured by TBARs and FRAP methods, respectively. Result: Our results showed that, TAC level in seminal plasma of control men was significantly higher than alcoholic men and had positive correlation with sperm count, motility and morphology. In contrast seminal and blood plasma MDA levels in control men were significantly lower than in alcoholic and smokers men and had negative correlation with sperm count, motility and morphology Conclusion: Thus, it seems that alcoholic and smoking strengthening decrease the antioxidant capacity of idiopathic infertile male and may prevent developing malfunction.
