Backgrounds: SARS-CoV-2 has a single-stranded RNA-plus genome (~30 kb) with five major open reading frames, which is encode nonstructural replicase polyproteins and structural proteins: Spike (S), Envelope (E), Membrane (M), and Nucleocapsid (N). Spike protein as known a causative ligand for mammalian cell’s angiotensin-converting enzyme 2 (ACE2) receptor which transmits the virus in to host cells. Due to the high expression of ACE and ACE2 receptor in various organs, the aim of this study is to investigation of correlation between ACE and ACE2 receptor with other proteins, which involved in the male infertility using by in silico analysis. Materials and Methods: In this study free web databases such as NCBI (https://www.ncbi.nlm.nih.gov) and MalaCards (https://www.malacards.org) and online proteins interaction data analyzer e.g., GeneMANIA server (https://genemania.org) were used. Results: Our studies showed that ACE and ACE2 receptors have a higher expression in testicular tissue. In other hand interaction between proteins showed these receptors have co-expression with Protamine 1/PRM1 and Protamine 2/ PRM2. Conclusion: Since Protamines are a special protein in the sperm cells, so defective of this protein cause the sperm abnormalities and affects in male infertility. We recommend, occupation of ACE and ACE2 receptors by SARS-CoV-2 may cause to verity of protamine expression level, which effect on male infertility risk.
