Background and Aim: CD44 is a cell surface glycoprotein, which is known as a cell adhesion molecule/CAM. It plays a major role in cell-cell adhesion, cell-substrate interaction, lymphocyte homing, idiopathic recurrent miscarriage, and tumor metastasis. On the other hand, malignant cells can initiate a tumor metastasis by sustaining proliferative signaling. Thus it is probable that some of the CD44 gene mutations can stimulate and sustain progression of cells through their growth and division cycles. The aim of this study is to investigate CD44-C353647G and -G187116A gene polymorphisms with benign prostatic hyperplasia by RFLP. Methods: The boiling DNA extraction performed from 150 patients and 70 normal control blood samples, which collected from Rouhani Hospital (Babol, Iran). The PCR- RFLP have been used from products of the C353647G (401 bp) and G187116A (694 bp), and digested by HinfI and HpaII restriction enzymes pattern, respectively. For visualizing the RFLP bonds, the 1% agarose gel electrophoresis has been used. Results: There are 3 types of bonds in C353647G genotyping with 121, 280 bp; 401, 121, 280 bp; and 401 for CC; CG; and GG genotypes, respectively. Also, we observed 3 types of bonds in G187116A genotyping with 453, 241 bp; 694, 453, 241 bp; and 694 for GG; GA; and AA genotypes, respectively. The frequency of alleles was almost significantly different (Pvalue = 0.09) in case and control groups. Conclusion: In spite of what our study showed, the frequency of allele might be more different in case and control groups if the number of sample increases. And so, these intron-SNPs of the CD44 as a tumor marker on the stability of transcript molecules; can be studied by other researchers.
