The green synthesis method has lately been widely employed in the creation of metal nano-crystallites due to its cheap cost and abundant supply such as. Acacia Senegal with silver iodide. A silver iodide substance is often utilized and may have antibacterial qualities The results of biological application (antibacterial) was employed in the current study to produce silver oxide nanocrystallites. X-ray Diffraction Spectroscopy (XRD), Atomic force microscopy (AFM), Scanning Electron Microscope (SEM), Fourier Transform-Infrared Spectroscopy (FTIR), Transmission Electron Microscope (TEM), and Ultraviolet–Visible Spectroscopy (UV-Vis) have all been used to examine the nanostructural properties and antibacterial activities of silver oxide (Ag2O and AgO) nano crystallite. The films are nano-crystallized in the cubic spinel configuration and semi-spherical form crystallites with great dispensability and homogeneity. The average value was 14.31 nm, and the highest value was 34.50 nm, and the root mean square was 4.00 nm, according to the findings of the acquired AFM pictures. Card number (01–1164) (2Ɵ, hkl) equal to (24.390°.112), Card number (01–1041) (2Ɵ, hkl) equal to (39.010°,420), and Card number (42–0874) (2Ɵ, hkl) equal to (46.120°,420) are all by XRD measured data. Silver oxide was confirmed by plasmon resonance peaks in the sample's UV–Vis spectrum at a wavelength of 218 nm. Additionally, the efficiency of biologically produced silver oxide nanoparticles (NPs) against four distinct dangerous bacterial species was investigated. The results showed that the biosynthesized silver oxide NPs were high effective against S. epidermidis and were least effective against S. aureus, E. coli, and Klebsiella sp.
