A noteworthy component in upgrading renewable energy conversion and storage device is the development of oxygen reduction reaction (ORR) electrocatalysts. Although micropore active sites sustain high ORR activity, meso-micropores play a crucial role in enhancing single-cell performance. Our breakthrough is that we prepared 0.2 mg cm-2 of AgCo-MoS2/rGO (abbreviated as ACMSr) electrocatalyst as the cathode catalyst loading and 0.2 mg cm-2 of Pt/C as the anode catalyst loading in the fuel cell test, and as a result, achieved peak power density and maximum current density of 221 mW cm-2 and 525.41 mA cm-2, respectively. Various parameters, such as relative humidity, operating temperature, amount of injected oxygen, and back pressure, affect the performance of the fuel cell. A decrease in the humidity of the cathode negatively impacts the performance of the fuel cell. At temperatures of 65 to 75 °C, the fuel cell’s performance increased at various temperatures. The performance of the fuel cell is directly affected by the rate of injected oxygen through the amount of gas flow and indirectly by the flooding effect in the cathode and membrane humidity. These achieved data provide a suitable approach for the design and modeling of fuel cells.