In this study, Au/Fe nano-crystallites were obtained using two seed growth and solvothermal methods; and in the next stage, their biosensor features were evaluated in identifying albumins. Au and Fe nano-crystallites were separately synthesized and were tested in the form of composites with the matrix hexadecyl trimethyl ammonium bromide as samples. Then, in order to evaluate the geometric structure, nanostructure features and the role of localized surface plasmons, spectrums were measured for the samples by the use of visible-ultraviolet spectroscopy and images were taken by the use of electron transmission microscope. Then it was observed in the bovine blood serum, which is very similar to human blood serum, that sensors based on Au/Fe nano-crystallites were able to show the quantity of albumins in lower concentrations in the bovine blood serum. This can be considered as a good result for prevention of kidney diseases in humans.