Pseudomonas aeruginosa (P. aeruginosa) is a highly clinically important pathogen as it causes and chronic infections, especially in patients with damaged immune systems [1]. Moreover, P. aeruginosa can easily develop many mechanisms of resistance against antibiotics, leading to most antibiotics becoming useless. therefore, rapid and accurate identification of P. aeruginosa has important effects on the therapy and management of infectious diseases caused by P. aeruginosa [2]. Various methods have been developed for the detection of P. aeruginosa, such as enzyme-linked immunosorbent assay (ELISA), immunofluorescence, polymerase chain reaction (PCR), flow cytometry (FC), fluorescence microscopy (FM) [3]. Most of them are accurate but are accompanied by several problems in on-site detection, such as procedures being complicated and cumbersome, time is long, sensitivity is limited, highly sophisticated instruments and expensive, skilled manpower and the cost is high [2]. Therefore, to combat the aforementioned challenges, there is an urgent need for new powerful tools that are rapid and sensitive with the ability to detect P. aeruginosa on-site and that can guide the concerned person for taking appropriate control measures. In the past few years, aptasensors were used for detecting the type of some bacteria [4]. Among various detection techniques, electrochemical methods have been recognized as one of the most promising technologies due to their high sensitivity, fabrication simplicity, accuracy, cost-effectiveness, selection, the possibility of mass production, and ease of modification [5]. The present work aims are to develop a selective and sensitive analytical method for the accurate diagnosis of P. aeruginosa bacteria. Hereon we developed an innovative electrochemical aptasensor for detection of P. aeruginosa bacteria based on using two-dimensional material graphitic carbon nitride functionalized, as the electrochemical signal label to amplify signals. Based on this
