The main drawback of current antibiotic therapies
is the emergence and rapid increase in antibiotic
resistance. Nocardiae are aerobic, Gram-positive, catalasepositive,
non-motile actinomycetes. Nocardia brasiliensis
was reported as antibiotic producer. The purpose of the
study was to determine antibacterial activity of N.
brasiliensis PTCC 1422 against isolated Enterobacteriaceae
from urinary tract infections (UTIs). The common
bacteria from UTIs were isolated from hospital samples.
Antimicrobial susceptibility test was performed for the
isolated pathogens using Kirby–Bauer disk diffusion
method according to clinical and Laboratory Standards
Institute guideline. Antagonistic activity of N. brasiliensis
PTCC 1422 was examined with well diffusion methods.
Supernatant of N. brasiliensis PTCC 1422 by submerged
culture was analyzed with gas chromatography–mass
spectrometry. Isolated strains included Escherichia coli,
Klebsiella pneumoniae, Serratia marcescens and Proteus
mirabilis. The most common pathogen isolated was E. coli
(72.5 %). Bacterial isolates revealed the presence of high
levels of antimicrobial resistances to ceftriaxone and low