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Naser Jafari

Naser Jafari

Academic rank: Associate Professor
ORCID:
Education: PhD.
ScopusId:
Faculty: Science
Address: Department of biology, Faculty of basic sciences, University of Mazandaran, Babolsar, Iran, P.C: 47416-95447
Phone: 01135302451

Research

Title
Submitted in Partial fulfillment of the requirements for the degree of Master of Science in Plant Physiology
Type
Thesis
Keywords
Ascorbic acid, Mungbean, Water stress, Polyethylene glycol
Year
2023
Researchers Naser Jafari(PrimaryAdvisor)، Ibtihal Jebur Ali(Student)

Abstract

Drought in the world is one of the most important challenges facing countries, which causes many damages. Drought can cause the loss of natural resources, different ecosystems and water resources. It is also known that the occurrence of water shortage and drought can be an impact factor on human life. Drought can increase deaths and indigestion in the world by reducing food security because the most important factor in increasing food security is agriculture, which supplies food to different communities. For this reason, scientists are looking for solutions to increase food security and reduce the damages caused by drought. Using plants with high nutritional value or effective chemical compounds in stressful conditions can be a suitable solution to improve plant performance. It has been found that ascorbic acid has high antioxidant properties and can protect plants against stressful conditions and improve plant performance under stressful conditions. On the other hand, the mung bean plant is valuable in terms of food and contains high amounts of protein that can be cultivated as a nutritious plant. In the present study, the effects of ascorbic acid at concentrations of 50 and 100 mg/liter on two different varieties of Iraqi and Turkish mung bean plants were investigated under dry conditions. In this study, after washing and disinfecting, mung bean seeds were placed in a solution containing 50 and 100 mg/liter ascorbic acid for 24 hours and then dried at room temperature for 48 hours. In order to induce drought stress, PEG6000 or polyethylene glycol was used and stress conditions of -2, -4 and -8 times were induced to the seedlings. The seeds treated in ascorbic acid were placed in petri dishes containing polyethylene glycol and harvested after 10 days, and finally the appearance parameters such as fresh weight, root length, shoot and leaf area, as well as biochemical parameters such as the amount of plant pigments, Phenolic compounds, total sugar were measured. The results of the current research showed that increasing the stress level can cause negative effects on plant growth and reduce the plant's appearance parameters. It was found that only the parameters of root weight and number at the 2-bar level in the Iraqi variety and root and shoot length in the Turkish variety at the 2- and 4-bar levels showed better effects in the treatment with ascorbic acid 50 and 100 mg/liter. In addition, it was found that the Turkish variety performed better than the Iraqi variety. The results of the present study showed that increasing the level of dryness caused a decrease in RWC, plant pigments and flavonoids, but increased the level of total sugar, total protein and total phenol and antioxidant properties. It was observed that ascorbic acid 100 mg/liter had better effects on RWC, photosynthetic pigments and flavonoids, especially in Turkish variety. In addition, treatment of Iraqi variety with ascorbic acid 50 mg/liter showed more phenol content than other treatments. Also, it was found that the ascorbic acid treatments of 50 mg/liter and 100 mg/liter in the Iraqi variety had the least and the most effect on the amount of protein compared to other seedlings, respectively. All the seedlings of the Turkish variety showed a significant increase in the amount of total sugar, but in the Iraqi variety only dry level of -8 Bar in the treatments with ascorbic acid 50 mg/liter and dry level of -4 and -8 Bar with ascorbic acid 100 mg/liter showed a significant increase. It was found that Turkish cultivars showed more antioxidant properties compared to Iraqi cultivars, and this increase was greater in the treatment of 100 mg/liter of ascorbic acid than in the treatment of 50 mg/liter.