1403/01/10
جهانبخش رئوف

جهانبخش رئوف

مرتبه علمی: استاد
ارکید:
تحصیلات: دکترای تخصصی
اسکاپوس:
دانشکده: دانشکده شیمی
نشانی:
تلفن: 01135302392

مشخصات پژوهش

عنوان
A Novel Electrochemical Genosensor Based on Banana and Nano-Gold Modified Electrode Using Tyrosinase Enzyme as Indicator
نوع پژوهش
JournalPaper
کلیدواژه‌ها
Hybridization Biosensor, Tyrosinase, Gold Nanoparticle, Banana Modified Carbon Paste Electrode.
سال
2015
مجله JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
شناسه DOI
پژوهشگران Jahan Bakhsh Raoof ، Maryam Asghary ، Ezat Hamidi-Asl ، Reza Ojani

چکیده

The electrochemical behavior of the tyrosinase enzyme at the surface of two electrodes, carbon paste electrode (CPE) and nano-gold modified carbon paste electrode (NGCPE), has been studied by cyclic voltammetry. Tyrosinase showed one oxidation peak (around+0.85 V) and one reduction peak at+0.40 V versus Ag AgCl KCl (3 M). To calculate the values of and, ks, the effect of potential scan rate on the electrochemical properties of tyrosinase was investigated. Cyclic voltammetry and UV-vis absorption techniques were used for the study of interaction between DNA and tyrosinase. The cyclic voltammogram of tyrosinase was obtained in the presence of different types of DNA bases for the study of tyrosinase-DNA binding. The results showed that the hydrogen binding and electrostatic interactions were important interaction mode. Moreover, a variation in tyrosinase signals intensity regarding the interaction to ssDNA and dsDNA was observed. The selectivity of the biosensor was studied using noncomplementary oligonucleotides. Finally, banana modified carbon paste electrode was also prepared to investigate the interaction of banana’s tyrosinase with DNA. The limit of detection for DNA probe was calculated 0.33 pM by using the oxidation signal of accumulated tyrosinase in NGCPE.