1403/01/09
جهانبخش رئوف

جهانبخش رئوف

مرتبه علمی: استاد
ارکید:
تحصیلات: دکترای تخصصی
اسکاپوس:
دانشکده: دانشکده شیمی
نشانی:
تلفن: 01135302392

مشخصات پژوهش

عنوان
Construction of a highly sensitive signal-on aptasensor based on gold nanoparticles/functionalized silica nanoparticles for selective detection of tryptophan
نوع پژوهش
JournalPaper
کلیدواژه‌ها
Tryptophan; Hemin; Signal-on aptasensor; Functionalized silica nanoparticles; Gold nanoparticles
سال
2017
مجله ANALYTICAL AND BIOANALYTICAL CHEMISTRY
شناسه DOI
پژوهشگران Ayemeh Baghery Hashkavayi ، Jahan Bakhsh Raoof ، Reza Ojani

چکیده

In this work, a highly sensitive, low-cost and label-free aptasensor based on signal-on mechanisms of response was developed by immobilizing the aptamer on gold nanoparticles (AuNPs)/amine-functionalized silica nanoparticle (FSN)/screen printed electrode (SPE) surface for highly selective electrochemical detection of Tryptophan (Trp). The hemin (Hem), which interacted with the guanine bases of the aptamer, worked as a redox indicator to generate a readable electrochemical signal. The changes in the charge transfer resistance have been monitored using the voltammetry and electrochemical impedance spectroscopic (EIS) techniques. The peak current of Hem linearly increased with increasing concentration of Trp, in differential pulse voltammetry, from 0.06–250 nM with a detection limit of 0.026 nM. Also, the results obtained from EIS studies showed that the Trp was detected sensitively with the fabricated aptasensor in the range of 0.06–250 nM. The detection limit is 0.01 nM, and much lower than that obtained by most of the reported electrochemical methods. The usage of aptamer as a recognition layer led to a sensor with high affinity for Trp, compared with control amino acids of tyrosine, histidine, arginine, lysine, valine, methionine. The usability of the aptasensor was successfully evaluated by determination of Trp in a human blood serum sample. Thus, the sensor could provide a promising plan for the construction of aptasensors.