1403/01/10
جهانبخش رئوف

جهانبخش رئوف

مرتبه علمی: استاد
ارکید:
تحصیلات: دکترای تخصصی
اسکاپوس:
دانشکده: دانشکده شیمی
نشانی:
تلفن: 01135302392

مشخصات پژوهش

عنوان
A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles
نوع پژوهش
JournalPaper
کلیدواژه‌ها
Magnetic beads; ELISA; Sandwich hybridization; Microfluidic platform; PCR products
سال
2015
مجله ELECTROANALYSIS
شناسه DOI
پژوهشگران Ezat Hamidi-Asl ، Mohammad Saeid Hejazi ، Simin Sharifi ، Seyed Mahdi Golabi ، Ilaria Palchetti ، Marco Mascini ، Jahan Bakhsh Raoof

چکیده

The development of an electrochemical genosensor involving DNA biotinylated capture probe immobilized on streptavidin coated paramagnetic beads and microfluidic based platform for the detection of P53 gene PCR product is reported. The novelty of this work is the combination of a sensitive electrochemical platform and a proper microfluidic system with a simple and effective enzyme signal amplification technology, ELISA, for detection of target DNA sequence and single nucleotide mutation in p53 tumor suppressor gene sequence. The biosensor has been applied to detect the PCR amplified samples and the results shows that it can discriminate successfully perfect matched DNA from mutant form.