1403/01/10

محمد جواد چایچی

مرتبه علمی: استاد
ارکید:
تحصیلات: دکترای تخصصی
اسکاپوس:
دانشکده: دانشکده شیمی
نشانی:
تلفن: 9113120679

مشخصات پژوهش

عنوان
Evaluation of Luminol Chemiluminescence Based on Simultaneous Introducing of Coumarin Derivatives as Green Fluorophores and Chitosan-Induced Au/Ag Alloy Nanoparticle as Catalyst for the Sensitive Determination of Glucose
نوع پژوهش
JournalPaper
کلیدواژه‌ها
Chemiluminescence, Luminol, Fluorophore, Au/Ag alloy NPs, Glucose
سال
2015
مجله JOURNAL OF FLUORESCENCE
شناسه DOI
پژوهشگران Mohammad Javad Chaichi ، seyedeh olia Alijanpour ، Sakineh Asghari ، shima Shadlou

چکیده

We report herein the development of a novel chemiluminescence system based on simultaneous introducing of synthetic coumarin derivatives and chitosan-induced Au/Ag alloy NPs on the luminol CL system and suggest how it may be useful for determination of glucose. Chitosaninduced Au/Ag nanoalloys in the coumarin derivatives intensified-luminol CL system, in addition to catalyze CL reaction can make a change in the process of coumarin derivatives effect as fluorophore on the luminol CL system. This phenomenon is caused by interaction between active functional groups of coumarin derivatives and chitosan. The interaction strength depends on the coumarin derivatives’ structure and their substituents. Considering the inevitable trend luminol radical and superoxide anion radical to absorption on the surface of the embedded Au/Ag nanoalloy in the chitosan matrix, it can be concluded that chitosan acts as a platform for all reagents involved in the CL reaction including coumarin derivatives, Au/Ag nanoalloy and luminol, and electron-transfer taking place on it; Placing all chemiluminescent reagents together on the chitosan network can lead to a powerful CL due to increasing rigidity of CL system. The most efficient coumarin derivative on the Au/Ag nanoalloyfluorophore-luminol-H2O2 CL system, in relation to interaction capability with chitosan’ functional groups, was selected and the CL condition in presence of it was optimized. Whereas the glucose oxidase-mediated oxidation of glucose yields gluconic acid and H2O2, under optimum condition the most efficient CL system was applied to detection of glucose due to enzymatically production of hydrogen peroxide. The linear response range of 1.5×10−6–5.0×10−3 M and the detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) of 7.5×10−7Mwas found for the glucose standards. Also, the developed method was successfully applied to determination of glucose in real serum and urine samples of d