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Bagher Seyedalipour

Bagher Seyedalipour

Academic rank: Associate Professor
ORCID: http://orcid.org/0000-0002-3854-9328
Education: PhD.
ScopusId: https://www.scopus.com/authid/detail.uri?authorId=56725735600
Faculty: Science
Address: Department of Cellular and Molecular, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Phone: 01135302405

Research

Title
Moderately Halophilic Bacteria and Their Industrially Important Enzymes from the Ancient Ecosystem Badab-e Surt
Type
JournalPaper
Keywords
Badab-e Surt, extracellular enzymes, Gammaproteobacteria, lipase, moderately halophilic bacteria
Year
2019
Journal Industrial Biotechnology
DOI
Researchers Leila Satari Faghihi ، Bagher Seyedalipour ، salman ahmady asbchin ، Gholam Hossein Riazi

Abstract

The stability of enzymes produced by halophilic bacteria under harsh conditions makes them appropriate candidates as biocatalysts. The present study focuses on hydrolysis enzymes production by halophilic bacteria from Badab-e Surt springs and investigates their lipolytic activity. First, 24 bacterial isolates were screened and selected based on different morphological and biochemical properties. Next, the production of extracellular enzymes, such as lipase, protease, amylase, and other hydrolyses were studied. Five isolates with the maximum lipolytic activity were then selected for 16S rRNA gene analysis. Phylogenetic analysis of isolates showed that they belong to the class of Gamma-proteobacteria, and were most similar to the genera Halomonas, Marinobacter, Vibrio, and Idiomarina. The study of optimum growth conditions for isolates indicated that all strains were classified as moderately halophilic bacteria. The lipase-specific activity of the strains S-3, S-10, S-11, S-14, and S-18 were 63.84, 98.04, 154.63, 65.35, and 71.98 Units/mg of protein, respectively. Enzyme activity of isolates proved that isolates could be attractive options for biotechnological applications under the enzymatic processes